| Branch Name | First Stop | Start Time | Last Stop | End Time | Travel Time |
|---|
|
| ↑Broadmeadows Station | Matthews Ave on Dromana Ave | 1:09:00AM (following day) | Pascoe Vale Rd on Broadmeadows Station | 1:24:00AM | 15:00 |
| ↓City | Mt Alexander Rd on 27-Essendon Tram Depot | 2:05:00AM (following day) | Queen St on Little Collins St | 2:17:00AM | 12:00 |
| ↑Broadmeadows Station | Matthews Ave on Dromana Ave | 2:09:00AM (following day) | Pascoe Vale Rd on Broadmeadows Station | 2:24:00AM | 15:00 |
| ↑Broadmeadows (via Niddrie) | Queen St on Flinders Lane | 2:20:00AM (following day) | Pascoe Vale Rd on Broadmeadows Station | 3:10:00AM | 50:00 |
| ↓City (via Niddrie) | Pascoe Vale Rd on Broadmeadows Station | 2:25:00AM (following day) | Queen St on Little Collins St | 3:15:00AM | 50:00 |
| ↑Broadmeadows (via Niddrie) | Queen St on Flinders Lane | 3:20:00AM (following day) | Pascoe Vale Rd on Broadmeadows Station | 4:10:00AM | 50:00 |
| ↓City (via Niddrie) | Pascoe Vale Rd on Broadmeadows Station | 3:25:00AM (following day) | Queen St on Little Collins St | 4:15:00AM | 50:00 |
| ↑Broadmeadows (via Niddrie) | Queen St on Flinders Lane | 4:20:00AM (following day) | Pascoe Vale Rd on Broadmeadows Station | 5:10:00AM | 50:00 |
| ↓City (via Niddrie) | Pascoe Vale Rd on Broadmeadows Station | 4:25:00AM (following day) | Queen St on Little Collins St | 5:15:00AM | 50:00 |