| Branch Name | First Stop | Start Time | Last Stop | End Time | Travel Time |
|---|
|
| →31E - Union Station | Georgetown GO Bus | 12:05:00AM | Union Station Bus Terminal | 1:35:00AM | 1:30:00 |
| ←31L - Bramalea GO | Union Station Bus Terminal | 12:41:00AM | Bramalea GO Bus | 1:21:00AM | 40:00 |
| ←31K - Mount Pleasant GO | Union Station Bus Terminal | 12:51:00AM | Mount Pleasant GO Bus | 1:51:00AM | 1:00:00 |
| ←31X - Mount Pleasant GO | Union Station Bus Terminal | 1:31:00AM | Mount Pleasant GO Bus | 2:41:00AM | 1:10:00 |
| ←31E - Georgetown GO | Union Station Bus Terminal | 2:31:00AM | Georgetown GO Bus | 3:56:00AM | 1:25:00 |
| →31E - Union Station | Georgetown GO Bus | 4:55:00AM | Union Station Bus Terminal | 6:30:00AM | 1:35:00 |
| →31K - Union Station | Mount Pleasant GO Bus | 5:35:00AM | Union Station Bus Terminal | 6:35:00AM | 1:00:00 |
| →31X - Union Station | Mount Pleasant GO Bus | 5:50:00AM | Union Station Bus Terminal | 7:10:00AM | 1:20:00 |
| →31F - Union Station | Georgetown GO Bus | 5:55:00AM | Union Station Bus Terminal | 7:15:00AM | 1:20:00 |
| →31K - Union Station | Mount Pleasant GO Bus | 6:30:00AM | Union Station Bus Terminal | 7:30:00AM | 1:00:00 |
| →31K - Union Station | Mount Pleasant GO Bus | 6:45:00AM | Union Station Bus Terminal | 7:45:00AM | 1:00:00 |
| →31L - Union Station | Bramalea GO Bus | 6:55:00AM | Union Station Bus Terminal | 7:40:00AM | 45:00 |
| ←31A - U of Guelph | Union Station Bus Terminal | 7:46:00AM | University of Guelph | 10:11:00AM | 2:25:00 |
| ←31L - Bramalea GO | Union Station Bus Terminal | 7:51:00AM | Bramalea GO Bus | 8:31:00AM | 40:00 |
| →31E - Union Station | Georgetown GO Bus | 11:00:00PM | Union Station Bus Terminal | 12:35:00AM | 1:35:00 |
| →31E - Union Station | Georgetown GO Bus | 12:05:00AM (following day) | Union Station Bus Terminal | 1:35:00AM | 1:30:00 |
| ←31L - Bramalea GO | Union Station Bus Terminal | 12:41:00AM (following day) | Bramalea GO Bus | 1:21:00AM | 40:00 |
| ←31K - Mount Pleasant GO | Union Station Bus Terminal | 12:51:00AM (following day) | Mount Pleasant GO Bus | 1:51:00AM | 1:00:00 |
| ←31E - Georgetown GO | Union Station Bus Terminal | 1:01:00AM (following day) | Georgetown GO Bus | 2:31:00AM | 1:30:00 |
| ←31X - Mount Pleasant GO | Union Station Bus Terminal | 1:31:00AM (following day) | Mount Pleasant GO Bus | 2:41:00AM | 1:10:00 |
| ←31E - Georgetown GO | Union Station Bus Terminal | 2:01:00AM (following day) | Georgetown GO Bus | 3:26:00AM | 1:25:00 |
| ←31E - Georgetown GO | Union Station Bus Terminal | 2:31:00AM (following day) | Georgetown GO Bus | 3:56:00AM | 1:25:00 |
| →31E - Union Station | Georgetown GO Bus | 4:00:00AM (following day) | Union Station Bus Terminal | 5:30:00AM | 1:30:00 |
| →31F - Union Station | Georgetown GO Bus | 4:40:00AM (following day) | Union Station Bus Terminal | 5:50:00AM | 1:10:00 |
| →31L - Union Station | Bramalea GO Bus | 5:15:00AM (following day) | Union Station Bus Terminal | 5:55:00AM | 40:00 |
| ←31 - U of Guelph | Union Station Bus Terminal | 5:46:00AM (following day) | University of Guelph | 8:31:00AM | 2:45:00 |
| ←31M - U of Guelph | Church St. E. (Brampton Bus Terminal) on Main St. N. | 6:10:00AM (following day) | University of Guelph | 7:50:00AM | 1:40:00 |
| ←31M - U of Guelph | Church St. E. (Brampton Bus Terminal) on Main St. N. | 6:30:00AM (following day) | University of Guelph | 8:10:00AM | 1:40:00 |