| Branch Name | First Stop | Start Time | Last Stop | End Time | Travel Time |
|---|
|
| ↓65e - Union Station | East Gwillimbury GO Bus | 5:05:00AM | Union Station Bus Terminal | 6:45:00AM | 1:40:00 |
| ↓65e - Union Station | East Gwillimbury GO Bus | 6:10:00AM | Union Station Bus Terminal | 7:50:00AM | 1:40:00 |
| ↑65c - Aurora Go | Union Station Bus Terminal | 7:07:00AM | Aurora GO Bus | 7:52:00AM | 45:00 |
| ↑65e - East Gwillimbury Go | Union Station Bus Terminal | 11:27:00PM | East Gwillimbury GO Bus | 1:07:00AM | 1:40:00 |
| ↑65e - East Gwillimbury Go | Union Station Bus Terminal | 12:12:00AM (following day) | East Gwillimbury GO Bus | 1:47:00AM | 1:35:00 |
| ↓65 - Union Station | East Gwillimbury GO Bus | 12:25:00AM (following day) | Union Station Bus Terminal | 1:30:00AM | 1:05:00 |
| ↑65e - East Gwillimbury Go | Union Station Bus Terminal | 2:12:00AM (following day) | East Gwillimbury GO Bus | 3:42:00AM | 1:30:00 |